《植物生理学报》 2011, 47(8): 771-776

美洲黑杨与大青杨杂种无性系耐寒性的初步研究

江锡兵¹, 郭斌¹, 宋跃朋¹, 马开峰¹, 安新民¹, 张志毅^1,*, 史志伟², 徐兰丽², 张有慧³

1北京林业大学林木育种国家工程实验室, 林木花卉遗传育种教育部重点实验室, 国家林业局树木花卉育种与生物工程重 点开放实验室, 北京100083; 2河北省平泉县林业局, 河北平泉067500; 3山东省冠县国营苗圃, 山东冠县252500

通信作者：张志毅；E-mail: zhangzy209@163.com；Tel: 010-62336248

摘　要：

以美洲黑杨与大青杨杂种无性系1 年生扦插苗为材料, 测定其低温胁迫中多个生理指标的变化。结果表明, 6个杂种 无性系SOD、POD和APX活性在低温胁迫中均表现出先上升后下降的趋势, 而电解质渗漏率、活性氧含量、CAT和GR活 性保持逐渐升高的趋势。无性系间差异显著, 其中DU146和DU147电解质渗漏率和活性氧含量最低, 而5种抗氧化酶活性 均最高, 表明其具有较强的耐寒性; 而DU135和DU150电解质渗漏率和活性氧含量最高, 而5种抗氧化酶活性均最低, 表明 其耐寒性差。

关键词：杨树; 杂种无性系; 低温; 耐寒性

收稿：2011-03-09   修定：2011-07-11

资助：国家林业公益性行业科研专项经费项目(201004009)。

Elementary Study on Cold-Tolerance of Hybrid Clones of Populus deltoides Bartr. × P. ussuriensis Kom.

JIANG Xi-Bing¹, GUO Bin¹, SONG Yue-Peng¹, MA Kai-Feng¹, AN Xin-Min¹, ZHANG Zhi-Yi^1,*, SHI Zhi-Wei², XU Lan-Li², ZHANG You-Hui³

¹National Engineering Laboratory for Tree Breeding, NDRC, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, Tree and Ornamental Plant Breeding and Biotechnology Laboratory, SFA, Beijing Forestry University, Beijing 100083, China; ²Forest Administration of Pingquan County, Hebei, Pingquan 067500, China; ³National Nursery of Guanxian County, Shandong, Guanxian 252500, China

Corresponding author: ZHANG Zhi-Yi; E-mail: zhangzy209@163.com; Tel: 010-62336248

Abstract:

One year old cuttings of hybrid clones of P. deltoides×P. ussuriensis were exposed to low temperatures in this work, and to determine changes of several physiological indices. The results indicated that activities of SOD, POD and APX in the six hybrid clones increased firstly and then declined under low temperature stress, whereas rates of electrolyte leakage, contents of O₂^-, and activities of CAT and GR kept gradually increasing trends. There were significant differences in these indices among the six clones, rates of electrolyte leakage and contents of O₂^- in clone DU146 and DU147 were the lowest, the activities of five antioxidant enzymes in clone DU146 and DU147 were the highest, these indicated that DU146 and DU147 had a strong resistance to cold, whereas clone DU135 and DU150 had poor cold-tolerance.

Key words: Poplar; hybrid clone; low temperature; cold-tolerance